Anti -PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| PTEN (also know as MMAC1) was recently identified as a candidate tumor suppressor gene. Deletions or mutations within the PTEN gene have been observed in a variety of tumor cell lines and cancer cell types. Based on PTEN’s structural features, the protein was originally thought to be a protein tyrosine phosphatase. However, recent evidence indicates that PTEN is actually a lipid phosphatase rather that a protein tyrosine phosphatase. In fact, the target substrate for PTEN is phosphatidylinositol-3,4,5-triphosphate (Ptd-Ins(3,4,5)P3). Ptd-Ins(3,4,5)P3 is a critical second messenger involved in cell signaling and growth control. Ptd-Ins(3,4,5)P3 is produced by phosphorylation of Ptd-Ins(4,5)P2 by PI 3-kinase in response to stimulation by various ligands. By dephosphorylating Ptd-Ins(3,4,5)P3, PTEN functions to “turn off” the Ptd-Ins(3,4,5)P3 pathway and thereby suppress cellular proliferation. | | | Catalog # | P9182-02 | | Applications | Suitable for use in ELISA, Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 0.1–1.0ug/ml | | Western Blot: 1–3ug/ml | | | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | A 22 amino acid synthetic peptide derived form the carboxy-terminus of the human PTEN protein. | | Specificity | Detects the ~55kD PTEN protein. Will recognize both endogenous PTEN and transfected, overexpressed PTEN. Species crossreactivity: Human and mouse PTEN proteins. Predicted to crossreact with canine based on sequence homology and differs by only one amino acid from the rat PTEN protein. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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