Anti -PTEN Tumor Suppressor Protein Tumor Suppressor Protein (MMAC1, Phosphatase and
Tensin, TEP1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| PTEN (also know as MMAC1) was recently identified as a candidate tumor suppressor gene. Deletions or mutations within the PTEN gene have been observed in a variety of tumor cell lines and cancer cell types. Based on PTEN’s structural features, the protein was originally thought to be a protein tyrosine phosphatase. However, recent evidence indicates that PTEN is actually a lipid phosphatase rather that a protein tyrosine phosphatase. In fact, the target substrate for PTEN is phosphatidylinositol-3,4,5-triphosphate (Ptd-Ins(3,4,5)P3). Ptd-Ins(3,4,5)P3 is a critical second messenger involved in cell signaling and growth control. Ptd-Ins(3,4,5)P3 is produced by phosphorylation of Ptd-Ins(4,5)P2 by PI 3-kinase in response to stimulation by various ligands. By dephosphorylating Ptd-Ins(3,4,5)P3, PTEN functions to “turn off” the Ptd-Ins(3,4,5)P3 | | | pathway and there by suppress cellular proliferation. | | | Catalog # | P9182-02A | | Applications | Suitable for use in Western Blot and ELISA. Other applications have not been tested. | | Recommended Dilution | Western Blot: 1-3ug/ml | | ELISA: 0.1-1.0ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 6A172 | | Host | Mouse | | Source | Human | | Concentration | 0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide | | Purity | Purified by Protein A chromatography | | Immunogen | Recombinant protein derived from the PTEN protein. | | Specificity | This antibody detects the ~55kD PTEN protein. The antibody will recognize both endogenous PTEN and transfected, overexpressed PTEN. Reacts specifically with the human PTEN protein. Reactivity with other species has not been tested. Reactivity was confirmed with cell lines which contain endogenous PTEN (NIH3T3, K652, and DU145 prostate cancer line) as well as transfected cell lines (Rat1 and 293T cells). No cross-reactivity to any other proteins has been detected. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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