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You are here:Home » Antibodies » Abs to Tumor Suppressors » Anti -PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)

Anti -PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit B IP IH FC IF
Catalog #P9182-02C
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Immunoprecipitation: 1:50
Flow Cytometry: 1:20
Immunohistochemistry: 1:50
Immunfluorescence: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control Testing Immunoblot Analysis A 1:500 dilution of the lot detected endogenous PTEN in lysates from MCF7 cells. Immunoprecipitation: A 1:50 dilution of this antibody immunoprecipitated PTEN from MCF-7 cell lysate using protein G:agarose beads. Flow Cytometry: A 1:20 dilution of this antibody stained cells for intracellular staining for flow cytometry analysis. Immunohistochemistry: A 1:50 dilution of this antibody was used for detection of PTEN in immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma. Immunfluorescence Staining: 1:50 dilution of this antibody was used for Immunfluorescence staining of paraformaldehyde fixed cells. Immunoblot Analysis: Lysate from MCF-7 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PTEN (1:500). Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PTEN (54kD).
Clone TypeMonoclonal
IsotypeIgG
Clone No7i53
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
ImmunogenSynthetic peptide corresponding to the C-terminal region of human PTEN.
SpecificityRecognizes the C-terminal domain of PTEN.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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