Anti -PTEN, Phosphorylated (Ser385) (Phosphatidylinositol-3,4,5-trisphosphate
3-phosphatase and Dual-specificity Protein Phosphatase PTEN, Mutated in Multiple Advanced
Cancers 1, Phosphatase and Tensin Homolog, MMAC1, TEP1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| The tumor suppressor phosphatase PTEN, a 55kD protein, regulates cell migration, growth, and survival by dephosphorylating phosphatidylinositol second messengers and signaling phosphoproteins. PTEN protein is composed of an N-terminal dual specificity phosphatase-like enzyme domain and C-terminal noncatalytic regulatory domain that contains multiple putative phosphorylation sites, which could play an important role in the control of its biological activity. Proper phosphorylation of PTEN by CK2 is important for PTEN protein stability to proteasome-mediated degradation. The phosphorylation sites in PTEN are located within a C-terminal cluster of Ser/Thr residues. The CK2 phosphorylation sites were identified as PTEN residues Ser-370, Ser-380, Thr-382, Thr-383, and Ser-385. | | | Catalog # | P9182-02R | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:5000-1:20,000 | | Western Blot: 1:500-1:1000 | | Immunohistochemistry: 1:200 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 2D60 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic phosphopeptide close to C-terminus of human PTEN at the phosphorylation site of Serine 385 (TTDpSDPE). | | Specificity | Recognizes human PTEN when Phosphorylated at Ser385. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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