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You are here:Home » Antibodies » Abs to Tumor Suppressors » Anti -PTEN Tumor Suppressor Protein, CT (MMAC1, Phosphatase and Tensin, TEP1)

Anti -PTEN Tumor Suppressor Protein, CT (MMAC1, Phosphatase and Tensin, TEP1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC FC
PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k”/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PD biding sequence (5). Phosporylation of the tail of the protein will suppress its activity and modify its conformation to a “closed” form an “open” form (5). Mutations in PTEN have been linked to glioblastoma, melanoma and prostate cancer.
Catalog #P9182-04Y
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:20
Western Blot: 1:500
Immunoprecipitation: 1:50
Immunohistochemistry: immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma using anti-PTEN (C-term) RabMAb 1:50
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlWestern Blot: MCF7 cell lysate
Immunohistochemistry: Human thyroid gland carcinoma (Paraffin-embedded)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k334
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in the C-terminal of human PTEN
SpecificityRecognizes human PTEN (Mr 54kD). Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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