| PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k”/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PD biding sequence (5). Phosporylation of the tail of the protein will suppress its activity and modify its conformation to a “closed” form an “open” form (5). Mutations in PTEN have been linked to glioblastoma, melanoma and prostate cancer. |
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| Catalog # | P9182-04Y |
| Applications | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:20 |
| Western Blot: 1:500 |
| Immunoprecipitation: 1:50 |
| Immunohistochemistry: immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma using anti-PTEN (C-term) RabMAb 1:50 |
| Immunocytochemistry: 1:50 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Western Blot: MCF7 cell lysate |
| Immunohistochemistry: Human thyroid gland carcinoma (Paraffin-embedded) |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 6k334 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. |
| Purity | Supernatant |
| Immunogen | A synthetic peptide corresponding to residues in the C-terminal of human PTEN |
| Specificity | Recognizes human PTEN (Mr 54kD). Species Crossreactivity: Mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
