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You are here:Home » Antibodies » Abs to Disease Markers » Anti -PTEN Tumor Suppressor Protein, phosphorylated (Thr366/Ser370) (Phosphatase and Tensin Homolog, Bannayan Zonana Syndrome, BZS, MGC11227, Multiple Hamartoma (Cowden Syndrome), MHAM, Mutated in Mutiple Advanced Cancers 1, MMAC1, Phosphatidylinositol-3,4,5-tr

Anti -PTEN Tumor Suppressor Protein, phosphorylated (Thr366/Ser370) (Phosphatase and
Tensin Homolog, Bannayan Zonana Syndrome, BZS, MGC11227, Multiple Hamartoma (Cowden
Syndrome), MHAM, Mutated in Mutiple Advanced Cancers 1, MMAC1,
Phosphatidylinositol-3,4,5-tr

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an
N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PDZ biding sequence.Phosphorylation of the tail suppresses the activity of PTEN by controlling its recruitment into the PTEN- associated complex (4).PTEN is phosphorylated in vitro on Threonine 366 and Serine 370 by glycogen synthase kindase 3 (GSK3) and casein kinase 2 (CK2) respectively.Prior phosphorylation of PTEN at Serine 370 by CK2 strongly
increased its phosphorylation at Threonine 366 by GSK3, suggesting that the two may synergize.Generally, phosphorylation in the C-terminal tail of PTEN is thought to enhance stability and to decrease membrane localization and activity.However, phosphorylation at Threonine 366 is linked to destabilization of PTEN (5).
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Catalog #P9182-16L
Recommended DilutionWestern Blot: 1:10,000-1:20,000
Immunoprecipitation: 1:50
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H497
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA phospho-specific peptide corresponding to residues surrounding Threonine 366 and Serine 370 of human PTEN.
SpecificityRecognizes human PTEN when phosphorylated at Thr366 and Ser370.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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