Anti -PTEN Tumor Suppressor Protein, phosphorylated (Thr366/Ser370) (Phosphatase and
Tensin Homolog, Bannayan Zonana Syndrome, BZS, MGC11227, Multiple Hamartoma (Cowden
Syndrome), MHAM, Mutated in Mutiple Advanced Cancers 1, MMAC1,
Phosphatidylinositol-3,4,5-tr
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC |
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| PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an | | | N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PDZ biding sequence.Phosphorylation of the tail suppresses the activity of PTEN by controlling its recruitment into the PTEN- associated complex (4).PTEN is phosphorylated in vitro on Threonine 366 and Serine 370 by glycogen synthase kindase 3 (GSK3) and casein kinase 2 (CK2) respectively.Prior phosphorylation of PTEN at Serine 370 by CK2 strongly | | | increased its phosphorylation at Threonine 366 by GSK3, suggesting that the two may synergize.Generally, phosphorylation in the C-terminal tail of PTEN is thought to enhance stability and to decrease membrane localization and activity.However, phosphorylation at Threonine 366 is linked to destabilization of PTEN (5). | | | | | | Applications: | | | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | | Catalog # | P9182-16L | | Recommended Dilution | Western Blot: 1:10,000-1:20,000 | | Immunoprecipitation: 1:50 | | Immunohistochemistry: 1:100-1:250 | | Immunocytochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H497 | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA. | | Purity | Supernatant | | Immunogen | A phospho-specific peptide corresponding to residues surrounding Threonine 366 and Serine 370 of human PTEN. | | Specificity | Recognizes human PTEN when phosphorylated at Thr366 and Ser370. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -PTEN Tumor Suppressor Protein, phosphorylated (Thr366/Ser370) (Phosphatase and Tensin Homolog, Bannayan Zonana Syndrome, BZS, MGC11227, Multiple Hamartoma (Cowden Syndrome), MHAM, Mutated in Mutiple Advanced Cancers 1, MMAC1, Phosphatidylinositol-3,4,5-tr |
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