| Pituitary tumor-transforming gene 1 (PTTG-1) is an oncogene that is expressed in normal tissues containing proliferating cells and in tumors. PTTG is downstream of the MAP kinase cascade and its phosphorylation at Ser162 by MAPK in vitro. The phosphorylation plays an essential role in PTTG’s transactivation function. PTTG has a DNA-binding domain, and it activates c-Myc (an oncogene) transcription by binding to the promoter in a protein complex. Studies found that expression of human PTTG correlates with cell proliferation in a cell cycle-dependent manner and the association of hPTTG with Ku, the regulatory subunit of DNA-dependent protein kinase, is disrupted when a DNA double-strand breaks occurs. |
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| Catalog # | P9182-75 |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 0.1-1ug/ml |
| Western Blot: 2-4ug/ml. Recognizes only the 28kD band in MCF-7 cell lysate. |
| Immunohistochemistry: 1-2ug/ml in formalin-fixed, paraffin embedded tissues. For best results heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| I1904-47J: IgG, H&L (AP) Pab GtxRt |
| I1904-40D: IgG, H&L (FITC) Pab Gt xRb |
| I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide derived from the transactivation domain within the N-terminus of the human PTTG-1 protein |
| Specificity | Recognizes human PTTG-1. Species sequence homology: mouse PTTG-1. Reactivity confirmed with human MCF-7 cell lysate (Westerb Blot) and colon cancer tissue (IHC, paraffin). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
