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Anti -PUMA, alpha (p53 Upregulated Modulator of Apoptosis)
Anti -PUMA, alpha (p53 Upregulated Modulator of Apoptosis)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC FC |
|
| PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta. | | | Catalog # | P9198-01F | | Applications | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:30 | | Western Blot: 1:1000-5000 | | Immunoprecipitation: 1:50 | | Immunohistochemistry: 1:100-250 | | Immunocytochemistry: 1:250-500 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k335 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15 sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues in the C-term of human PUMA. | | Specificity | Recognizes human PUMA. Predicted to detect both isoforms based on sequence analysis. The isoform B is ~18kD and isoform A is ~24kD. Isoform B is primarily recognized on the western blot gel. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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