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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Pvu II

Pvu II

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Specifications

5'-C A G^C T G-3'
3'-G T C^G A C-5'
Catalog #P9390
SourceProteus vulgaris
Unit DefinitionOne unit is defined as the amount of PvuI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Concentration10u/ul
Dilution/Storage BufferSupplied as a liquid in10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
Supplied WithR1625 Restriction Enzyme Buffer A, 10X
Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9
R1625-02 Restriction Enzyme Buffer C, 10X Supplied as a liquid in 10mM Tris-HCl pH 7.5 10mM Magnesium chloride, 50mM sodium chloride, 0.1mg/ml BSA Inhibition Temperature 37°C.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Stable for at least 12 months
Double Digests
Restriction Enzyme Buffer A is provided to simplify buffer selection for double digests. 98% of Restriction Endonucleased work well in a 1x or 2x concentration of theRestriction Enzyme Buffer A.
Number of Recognition Sites
Lambda15
M13mp18/193 pBR322: 1
pUC18/192 pUC57 : 2
pTZ19R/U2
X1740
Thermal Inactivation
PvuI is inactivated by incubation at 80°C for 20min.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 10-fold overdigestion (15u/ug lambda DNA x 1 hours) with PvuII (see Star Activity).
Ligation/Recutting AssayAfter 10-fold overdigestion (5u/ug DNA x 2 hours) with PvuII, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.06uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of PvuI for 4 hours.
Star ActivityA large excess of enzyme (15u/ug DNA x 1 hours) may result in star activity. Star activity appears at 10-fold overdigestion (10 units x 1 hour).
Methylation EffectDam
EcoBIMay overlaps- not determined
Stability during Prolonged IncubationA minimum of 0.2units of PvuI are required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.


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