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Anti -Pyk2, CAKb, FAK2, phosphorylated (Tyr402)
Anti -Pyk2, CAKb, FAK2, phosphorylated (Tyr402)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | P9400-02D | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Quality Control Testing Immunoblot Analysis | 1:1000 dilutions of this lot detected phospho-Pyk2 (Tyr402) in RIPA lysate of Pyk2 transfected CEF cells. Lysates from untransfected (Lane 1) and Pyk2 transfected (lanes 2 and 3) CEF cells grown on fibronectin were resolved by electrophoresis, transferred to | | nitrocellulose, and probed with antiphospho-Pyk2 (Tyr402) for 2 hours at room temperature. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho-Pyk2 (Tyr402). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Pyk2. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.2, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Peptide corresponding to amino acid region encompassing the human and rat phospho-Pyk2, CAb, FAK2 (Tyr402). | | Specificity | Recognizes phospho-Pyk2, CAK , FAK2 (Tyr402). Species Cross-reactivity: Human and Rat tested. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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