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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -Pyk2, CAKb, FAK2, phosphorylated (Tyr402)

Anti -Pyk2, CAKb, FAK2, phosphorylated (Tyr402)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #P9400-02D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control Testing Immunoblot Analysis 1:1000 dilutions of this lot detected phospho-Pyk2 (Tyr402) in RIPA lysate of Pyk2 transfected CEF cells. Lysates from untransfected (Lane 1) and Pyk2 transfected (lanes 2 and 3) CEF cells grown on fibronectin were resolved by electrophoresis, transferred to
nitrocellulose, and probed with antiphospho-Pyk2 (Tyr402) for 2 hours at room temperature. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho-Pyk2 (Tyr402). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Pyk2.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.2, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponding to amino acid region encompassing the human and rat phospho-Pyk2, CAb, FAK2 (Tyr402).
SpecificityRecognizes phospho-Pyk2, CAK , FAK2 (Tyr402). Species Cross-reactivity: Human and Rat tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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