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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -Pyk2 (CAKbeta, cdk-activating Protein Kinase beta)

Anti -Pyk2 (CAKbeta, cdk-activating Protein Kinase beta)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP
Pyk2 is a protein tyrosine kinase related to Focal Adhesion Kinase (p125 FAK). Pyk2 is highly expressed in the central nervous system. It is phosphorylated on tyrosine residues in response to various stimuli that elevate intracellular calcium concentration, as well as by protein kinase C activation.
Catalog #P9400-10
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:2000. Detects Pyk2 in RIPA lysates from rat brain cytosol.
Immunoprecipitation: 10ul precipitates Pyk2 from 2mg RIPA lysates from rat brain cytosol.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.126
HostMouse
SourceRat
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide.
PurityAscites
ImmunogenGST fusion protein corresponding to aa5-416 of rat Pyk2.
SpecificityRecognizes rat Pyk2 at ~115kD. Does not recognize focal adhesion kinase (FAK). Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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