Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Antibodies-Signal Transduction » Anti -Pygopus Homolog 1 (Pygopus-1, PYGO1)

Anti -Pygopus Homolog 1 (Pygopus-1, PYGO1)


  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.


Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45kD, 419aa transcriptional coactivator. It contains four domains: an N-terminal NLS (aa35-41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa340-398). It is a nuclear protein that acts in concert with BCL-9 to retain b-catenin in the nucleus during Wnt-signaling. In particular, following b-catenin activation and translocation to the nucleus, b-catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, b-catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 is 87% aa identical to mouse Pygopus-1.
Catalog #P9452-45
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No11C46
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E.coli-derived recombinant human Pygopus-1 (aa1-419; Accession # Q9Y3Y4).
SpecificityRecognizes human Pygopus-1. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

External Links