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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Pyrophosphatase, Inorganic, Recombinant, E.coli, aa1-176, His-Tag (Cytosolic Inorganic Pyrophosphatase, Pyrophosphate PhosphohydrolaPse)

Pyrophosphatase, Inorganic, Recombinant, E.coli, aa1-176, His-Tag (Cytosolic Inorganic
Pyrophosphatase, Pyrophosphate PhosphohydrolaPse)

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Specifications

Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations.
Catalog #P9542-50C
SourceRecombinant corresponding to aa1-176 of E.coli ppa, fused with His-tag at N-terminus, expressed in E.coli.
AA sequenceMGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE
AAKAEIVASF ERAKNK
Molecular Weight~21.9kD (196aa), confirmed by MALDI-TOF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~21.9kD
SourceE.coli
Purity~95% (SDS-PAGE)
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris-HCl, pH 8, 1mM DTT, 50mM sodium chloride, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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