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Pyrophosphatase, Inorganic, Recombinant, E.coli, aa1-176, His-tag (Cytosolic Inorganic Pyrophosphatase, Pyrophosphate PhosphohydrolaPse)
Pyrophosphatase, Inorganic, Recombinant, E.coli, aa1-176, His-tag (Cytosolic Inorganic
Pyrophosphatase, Pyrophosphate PhosphohydrolaPse)
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| Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. | | | Catalog # | P9542-50C | | Source | Recombinant corresponding to aa1-176 of E.coli ppa, fused with His-tag at N-terminus, expressed in E.coli. | | AA sequence | MGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE | | AAKAEIVASF ERAKNK | | Molecular Weight | ~21.9kD (196aa), confirmed by MALDI-TOF | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~21.9kD | | Source | E.coli | | Purity | ~95% (SDS-PAGE) | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl, pH 8, 1mM DTT, 50mM sodium chloride, 10% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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