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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » PPA1, aa1-289, Recombinant, Human (Inorganic Pyrophosphatase)

PPA1, aa1-289, Recombinant, Human (Inorganic Pyrophosphatase)

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Specifications

Inorganic Pyrophosphatases are enzymes that catalyze the hydrolysis of pyrophosphate to two phosphate ions (1). This reaction is highly exergonic, and is utilized in many biochemical pathways, such as DNA synthesis (2) and bone formation (3), to render reactions effectively irreversible (4, 5). Likewise, inorganic pyrophosphatases can be coupled to unfavorable biochemical reactions in order to drive these reactions to completion, such as in the synthesis of activated sulfur donor 3-phosphoadenosine-5-phosphosulfate (PAPS) (6). Two inorganic pyrophosphatases, i.e. PPA1 and PPA2, are found in humans. PPA1 is highly active on pyrophosphate and is ubiquitously expressed (7).
Catalog #P9542-51G
SourceRecombinant corresponding to aa1-289 from human PPA1, fused with 6-His tag at C-terminal, expressed in E. coli.
Molecular Weight~33kD
Endotoxin Level 1EU/1ug (LAL)
Biological ActivityMeasured by its ability to hydrolyze pyrophosphate. The specific activity is >50,000 pmoles/min/ug, as measured under the described conditions.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight~33kD
SourceE. coli
Purity~85% (SDS-PAGE)
ConcentrationAs reported
FormSupplied as a liquid in Tris, sodium chloride, DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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