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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -Pyruvate Kinase, Tumor Type M2 (PKM2)

Anti -Pyruvate Kinase, Tumor Type M2 (PKM2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Pyruvate kinase, a glycolytic enzyme, catalyzes the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1). Studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4). A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4). These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4).
Catalog #P9545-31
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunohistochemistry (Paraffin): 1:100; Using a citrate unmasking buffer.
Immunohistochemistry (Frozen): 1:100; Using a 3% formaldehyde fixative.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the sequence of human PKM2 conjugated to KLH. Molecular Weight: 60kD.
SpecificityRecognizes endogenous levels of total human PKM2 protein at 60kD. Species Crossreactivity: monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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