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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Rab3C

Anti -Rab3C

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions.
Catalog #R0006-76
Three membrane proteins synaptosomal associated protein of 25kD (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion.
There exist over 40 Rab proteins that have been described in mammals. Rab 3C is found in brain, adrenal gland and pituitary.
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml; Detects a 22kD protein representing Rab 3C from AtT20 cell extract.
Optimal dilutions to be determined by the researcher.
Positive Control  
AtT20 cells
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide: MASAQDARFGQKDSSDQN-C, corresponding to amino acids 9-26 of Rat Rab3C.
SpecificityRecognizes rat Rab3C. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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