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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions.
Catalog #	R0006-76G
	Three membrane proteins, synaptosomal associated protein of 25kD (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion.
	There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml; Detects an ~29kD protein representing recombinant mouse Rab 3D. Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	As reported
Form	Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic Peptide: M(1)ASASEPPASPRDAADQN(18). Immunizing peptide corresponds to amino acid residues 1-18 from rat rab 3D protein. This sequence is completely conserved between the rat and mouse.
Specificity	Recognizes rat Rab3D and detects recombinant mouse rab 3D. Does not detect rab 3A, 3B, or 3C.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.