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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -B-Raf, Phosphorlyated (Thr401)

Anti -B-Raf, Phosphorlyated (Thr401)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP
B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.
Catalog #R0007-14
ApplicationsSuitable for use in Western Blot, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
Immunocytochemistry: 1:100
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E509
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA phospho specific peptide corresponding to residues surrounding threonine 401 of human B-Raf.
SpecificityRecognizes human B-Raf phosphorylated on threonine 401. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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