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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Rab5 (Member RAS Oncogene Family, Ras-related Protein Rab-5, Rab GTPase)

Anti -Rab5 (Member RAS Oncogene Family, Ras-related Protein Rab-5, Rab GTPase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Rab5 is a 24kD GTP-binding protein that regulates the fusion of plasma membrane-derived clathrin-coated vesicles with early endosomes and homotypic fusion among early endosomes. It is localized to the cytoplasmic side of the plasma membrane, clathrin-coated vesicles, and early endosomes. Rab5 is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of Rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form, which represents the active form of Rab5, associates with membrane and regulates vesicle docking and fusion. Studies using Rab5 mutant that hydrolysed xanthosine 5í-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 is postulated to determine the frequency of membrane docking and fusion events.
Applications:
Suitable for use in Western Blot, and Immunoprecipitation Other applications not tested.
Catalog #R0008
Recommended DilutionWestern Blot (ECL): 1:2000 dilution detected Rab5 in 20ug of mouse brain tissue extract by ECL
Immunoprecipitation: 1:100
Optimal dilutions to be determined by researcher.
Positive Control:
Mouse Brain Tissue Extract, Rat Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 50% glycerol, 0.09% azide.
PurityPurified by Protein A affinity chromatography
ImmunogenHuman Rab5 synthetic peptide (aa182-197), conjugated to KLH. This sequence is identical to canine Rab5 over these residues.
SpecificityRecognizes human Rab5 at ~26kD. An additional higher molecular mass band of unknown identity at ~45kD is detected in some samples on Western blots. Species Crossreactivity: Mouse, rat, bovine, canine, guinea pig, hamster and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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