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You are here:Home » Antibodies » Abs to Organelle Markers » Anti -Rab5A (Member RAS Oncogene Family, Ras-related Protein Rab-5A, Rab GTPase)

Anti -Rab5A (Member RAS Oncogene Family, Ras-related Protein Rab-5A, Rab GTPase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Rab5a (Ras-related protein Rab-5a) is a 24kD lipid-anchored GTP-binding protein and member of the small GTPase superfamily, expressed on the cytoplasmic side of the plasma membrane and early endosomes, which plays a key role as a rate-limiting component of the endocytic pathway.
Catalog #R0008-02A
Rab5a cycles between a GDP-bound inactive form and its GTP-bound active form, generated by GDP/GTP exchange factors. It is the active form of Rab5a which associates with the membrane and regulates endocytic uptake and transfer to early endosomes whilst the inactivated form, generated by GTP hydrolysis, interacts with Rab escort protein (REP) and GDP dissociation inhibitor (GDI) and dissociates from the cell membrane for recycling.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:2,000
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No10F347
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human Rab5a (aa1-215) purified from Escherichia coli.
SpecificityRecognizes human Rab5a. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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