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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IP IH IF

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components.
It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.
Catalog #	R0008-80
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (Frozen sections), Immunofluorescence and Immunomicroscopy. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:500
	Immunohistochemistry (frozen): 1:500
	Western Blot: 1:1000; Detects an ~26kD protein representing Rab 9 from K562 cell extract. Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4i367
Host	Mouse
Source	Canine
Concentration	As reported
Form	Supplied as a liquid in PBS with 0.05% sodium azide.
Purity	Ascites.
Immunogen	Recombinant full length protein (Canine).
Specificity	Recognizes canine rab9. Recognizes prenylated and non-prenylated rab9. Does not crossreact with other rab family members. Species Crossreactivity: Human, Rat, Bovine, Feline, Hamster and Primate.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.