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R0009-05 Rabbit Anti-Rab11A (RAB11A Member RAS Oncogene Family, Rab-11A, MGC1490, RAB11, Rab-11, Ras-related Protein Rab-11A, Ras-related Protein Rab11A, YL8)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Swiss Prot
P62491, Q15907
Isotype
IgG
Clone Number
11C1063(D4F5)
Grade
Purified
Applications
IC IF IP WB
Crossreactivity
Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes. Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain. Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting.

Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:2000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Arg30 of human Rab11. Species Sequence Homology: hamster, porcine; 100%.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified.
Specificity
Recognizes endogeneous levels of human Rab11 at ~25kD. Reacts with isoforms Rab11a and Rab11b. Species Crossreactivity: monkey, mouse and rat.
References
1. Ullrich, O. et al. (1996) J. Cell Biol. 135, 913-924. 2. Chen, W. et al. (1998) Mol. Biol. Cell 9, 3241-3257. 3. Lapierre, L.A. et al. (2003) Exp. Cell Res. 290, 322-331. 4. Khvotchev, M.V. et al. (2003) J. Neurosci. 23, 10531-10539. 5. Junutula, J.R. et al. (2004) J. Biol. Chem. 279, 33430-33437. 6. Hales, C.M. et al. (2001) J. Biol. Chem. 276, 39067-39075.
USBio References
No references available
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