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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28,000 Da.
Catalog #	R0009-83
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:1000
	Immunohistochemistry: 1:100-1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Clone No	8A246
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Ni-NTA purified recombinant Rab25 expressed in E. coli strain BL21 (DE3)
Specificity	Recognizes truncated Rab25 recombinant protein / ovary carcinoma / stomach carcinoma / breast rcinoma lysate (WB), human Rab25 recombinant protein (ELISA), esophagus carcinoma tissue (IHC).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.