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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -RAD51 (BRCC5, DNA Repair Protein RAD51 Homolog 1, E. coli RecA Homolog, HGNC:9817, HRAD51, HsRad51, HsT16930, RAD51, RAD51A, RecA Homolog (E. coli), RecA-like Protein)

Anti -RAD51 (BRCC5, DNA Repair Protein RAD51 Homolog 1, E. coli RecA Homolog, HGNC:9817,
HRAD51, HsRad51, HsT16930, RAD51, RAD51A, RecA Homolog (E. coli), RecA-like Protein)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Rad51 (a eukaryotic homologue of Escherichia coli RecA) is similar to RecA, both biochemically and structurally: It promotes homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57 and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C and RAD 51D). Recent studies suggested that Rad51 is involved not only in homologous recombination but also in cell proliferation regulation.
Catalog #R0451-01B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunoprecipitation (Native): 2ug/mg protein lysate. Use Protein G.
Western Blot: 1-2ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
SourceAscites
Positive ControlHeLa cells and testis
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F326
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See R0451-01BX
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant Rad51 protein. Cellular Localization: Nuclear
MW37-43kD.
SpecificityRecognizes human Rad51. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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