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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -RAD51 (BRCC5, DNA Repair Protein RAD51 Homolog 1, E. coli RecA Homolog, HGNC:9817, HRAD51, HsRad51, HsT16930, RAD51, RAD51A, RecA Homolog (E. coli), RecA-like Protein)

Anti -RAD51 (BRCC5, DNA Repair Protein RAD51 Homolog 1, E. coli RecA Homolog, HGNC:9817,
HRAD51, HsRad51, HsT16930, RAD51, RAD51A, RecA Homolog (E. coli), RecA-like Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Rad51 is a structural and functional homolog of Escherichia coli RecA, which is required for genetic recombination, DNA repair, and cellular proliferation. This 43kD protein plays an important role in the repair of DNA double-strand breaks caused by ionizing radiation or from the breakdown of stalled replication forks. It forms helical nucleoprotein filaments in which the DNA lies extended and underwound. Filaments form preferentially on tailed duplex DNA substrates at the chromosomal break sites. Rad51 expression is increased in transformed cells and in cancer cell lines. Rad51 is overexpressed in breast carcinomas. Aberrant overexpression of Rad51 is directly correlated with radiation resistance and chemotherapeutic drug resistance. This aberrant expression of Rad51 is also related to enhanced recombination and genomic instabilities in breast carcinoma cells.
Catalog #R0451-01E
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Immunoprecipitation: 10ug/IP Reaction
Optimal dilutions to be determined by the researcher
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A174
HostMouse
SourceHuman
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide
PurityPurified by Protein A chromatography
ImmunogenPurified human RAD51 protein.
SpecificityThis antibody recognizes the human RAD51 protein. It can be used to analyse the role of the hRAD51 protein in genetic recombination. Reactivity is confirmed with Hela cells via Western blot analysis. Some cross reactivity has been observed in calf and pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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