Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Western Blot: 0.1-0.3ug/ml, observed in mouse testis lysates on ~37kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-HLNHRYQGKFLNKNP, from mouse RSPH1, at the internal region of the protein (NP_543136.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse RSPH1. Species sequence homology: Bovine, human and rat.
References
1. Shetty J, Klotz KL, Wolkowicz MJ, Flickinger CJ, Herr JC. Radial spoke protein 44 (human meichroacidin) is an axonemal alloantigen of sperm and cilia. Gene. 2007 Jul 1;396(1):93-107. Epub 2007 Mar 23.