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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -Raf Kinase Inhibitor Protein (RKIP)

Anti -Raf Kinase Inhibitor Protein (RKIP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Catalog #R0495-30
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects RKIP in RIPA cells. A previous lot detected RKIP in RIPA ltsates from 3T3/A31 cells.
Immunoprecipitation: 4ug immunoprecipitates RKIP from 500ug of 3T3/A31 RIPA lysate.
Optimal dilutions to be determined by the researcher.
Positive Antigen ControlF4209: 3T3/A31 cell lysate. Add 2.5ul of 2-mercaptoethanol to 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane
for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenFusion Protein corresponding to full length rat RKIP (Raf kinase inhibitor protein).
SpecificityRecognizes rat RKIP, Mr 23kD. Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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