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Anti -Raf Kinase Inhibitor Protein (RKIP)
Anti -Raf Kinase Inhibitor Protein (RKIP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP |
|
| Catalog # | R0495-30 | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml detects RKIP in RIPA cells. A previous lot detected RKIP in RIPA ltsates from 3T3/A31 cells. | | Immunoprecipitation: 4ug immunoprecipitates RKIP from 500ug of 3T3/A31 RIPA lysate. | | Optimal dilutions to be determined by the researcher. | | Positive Antigen Control | F4209: 3T3/A31 cell lysate. Add 2.5ul of 2-mercaptoethanol to 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane | | for minigels. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%. | | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Fusion Protein corresponding to full length rat RKIP (Raf kinase inhibitor protein). | | Specificity | Recognizes rat RKIP, Mr 23kD. Species Crossreactivity: human and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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