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Anti -Raf Kinase Inhibitor Protein, CT (RKIP)
Anti -Raf Kinase Inhibitor Protein, CT (RKIP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP |
|
| Raf-1 phosphorylates and activates MEK-1, a kinase that activates the extracellular signal-regulated kinases (ERK). The Ras/Raf/MEK/ERK module is a ubiquitously expressed signaling pathway that conveys mitogenic and differentiation signals from the cell membrane to the nucleus1. This kinase cascade controls the proliferation and differentiation of different cell | | | types. The small G protein Ras is activated by several growth factor receptors, and when activated binds Raf-1 kinase with high affinity. This induces the recruitment of Raf-1 from the cytosol to the cell membrane and its subsequent activation. Activated Raf-1 then phosphorylates the kinase MEK, thus activating it. MEK in turn phosphorylates and activates ERK, the prototypic mitogen-activated protein kinase (MAPK). Activated ERKs can translocate to the nucleus and regulate gene expression by the phosphorylation of transcription factors. Raf kinase inhibitor protein (RKIP) is a Raf-1 interacting protein that inhibits the phophorylation and activation of MEK by Raf-1. RKIP is not a substrate for either Raf-1 or MEK, but competitively disrupts the interaction between these kinases. | | | Catalog # | R0495-52 | | Applications | Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. | | Recommended Dilution | Immunoprecipitation: 7-10ug/IP reaction | | Western Blot: 1-3ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6A175 | | Host | Mouse | | Source | Human | | Concentration | 0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide | | Purity | Purifed by Protein A chromatography | | Immunogen | Synthetic peptide derived from the C-terminal region of human RKIP protein. | | Specificity | Specific for the C-terminal region of the RKIP protein. On Western blots, it identifies a band at 30kDa in RKIP-transfected HEK293 lysates and 23kDa in HEK293 lysates or total mouse brain lysates. Reactivity has been confirmed with HEK 293 lysates. Immunoprecipitation tests with HEK293 cell preparations also yielded the ~23kD band. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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