| mTORC1 kinase complex is a critical component in regulating cell growth (1,2). Its activity is modulated by energy levels, growth factors and amino acids (3,4). Recent studies found that RagA, RagB, RagC and RagD, the four related GTPases, interact with raptor in mTORC1 (1,2). |
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| These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). |
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| Catalog # | R1030-50 |
| Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% |
| Tween-20 at 4°C with gentle shaking, overnight. |
| Immunoprecipitation: 1:50 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 10E46 |
| Host | Rabbit |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol. |
| Purity | Purified |
| Immunogen | Synthetic peptide derived from the sequence of human RagA protein (KLH-coupled). |
| Specificity | Recongnizes endogenous levels of total human RagA protein. It may also detect endogenous levels of total RagB protein. Species crossreactivity: mouse, rat, monkey. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
