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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Ran (RAN, TC4, ARA24, RAN, member RAS oncogene family)

Anti -Ran (RAN, TC4, ARA24, RAN, member RAS oncogene family)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #R1069-01A
Alternate Name RAN, TC4, ARA24, RAN, member RAS oncogene family
Domain Information smart00176: Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases
Gene Ontology Terms DNA metabolism; GTP binding; GTPase activity; RNA-nucleus export; chromatin; intracellular protein transport; mitotic spindle assembly; nuclear pore; nucleus; protein transporter activity; protein-nucleus export; regulation of cell cycle; signal transduction; small GTPase mediated signal transduction
Application(s)Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 25kD band observed in Jurkat and A431 lysates (predicted MW of 25kD according to NP_006316). Recommended for use at 1-3ug/ml.
Staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See R1069-01A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenAAQGEPQVQFKLV-C, N Terminus
SpecificityRecognizes Human, Mouse, Rat, canine RAN
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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