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You are here:Home » Antibodies » Abs to RANK » Anti -RANK (Receptor Activator of NFkB)

Anti -RANK (Receptor Activator of NFkB)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IP FC
Catalog #R1075-01A
ApplicationsSuitable for use in Flow Cytometry, ELISA and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 20ug/ml, 20ul/10e6 cells in 100ul
ELISA: 20ug/ml
Immunoprecipitation: 20ug/ml
Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents.F(ab')2 Goat anti-rat IgG: FITC (mouse adsorbed)
.F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)
Recommended Negative ControlRat IgG2a Negative Control: I1904-82D1
HybridomaCells from immunized lou rat were fused with cells of the mouse NS-1 myeloma cel line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2863
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRANK-Fc fusion protein.
SpecificityRecognizes mouse receptor activator of NF Kappa-B (RANK). RANK is a member of the tumor necrosis factor (TNF) receptor superfamily and plays an important role in lymph node development and osteoclast differentation.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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