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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E IP FC

Catalog #	R1075-01A
Applications	Suitable for use in Flow Cytometry, ELISA and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Flow Cytometry: 20ug/ml, 20ul/10e6 cells in 100ul
	ELISA: 20ug/ml
	Immunoprecipitation: 20ug/ml
	Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents	.F(ab')2 Goat anti-rat IgG: FITC (mouse adsorbed)
	.F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)
Recommended Negative Control	Rat IgG2a Negative Control: I1904-82D1
Hybridoma	Cells from immunized lou rat were fused with cells of the mouse NS-1 myeloma cel line.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	3H2863
Host	Rat
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	RANK-Fc fusion protein.
Specificity	Recognizes mouse receptor activator of NF Kappa-B (RANK). RANK is a member of the tumor necrosis factor (TNF) receptor superfamily and plays an important role in lymph node development and osteoclast differentation.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.