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You are here:Home » Antibodies » Abs to RANK » Anti -RANK (Tumor Necrosis Factor Receptor Superfamily Member 11A, Osteoclast Differentiation Factor Receptor, ODFR, Receptor Activator of NF-KB, CD265, TNFRSF11A)

Anti -RANK (Tumor Necrosis Factor Receptor Superfamily Member 11A, Osteoclast
Differentiation Factor Receptor, ODFR, Receptor Activator of NF-KB, CD265, TNFRSF11A)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP FC
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).
Catalog #R1075-03
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 5ug/10e6 cells
Western Blot: 1-2ug/ml, 97-120kD
Immunoprecipitation: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Postive ControlsActivated T cells or RAW (a mouse macrophage, monocyte)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4H239
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein containing extracellular domain of human RANK.
SpecificityRecognizes human RANK (Receptor Activator of NF-kB).
Species CrossreactivityMouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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