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You are here:Home » Antibodies » Abs to RANK » Anti -RANK (Receptor Activator of NF-kB) (PE)

Anti -RANK (Receptor Activator of NF-kB) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).
Catalog #R1075-07
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.25-1ug/1x10e6 cells
The epitoipe range recognized by R1075-07 is a potential cytoplasmic region as defined by uniprot.org. Refer to Bharti et al. publication and the bioinformatics data base for additional information in order to determine the suitablility for cell surface flow cytometry.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlRAW mouse cells, fixed and permeabilized with acetone, can be used as positive control.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No4H238
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
PurityPurified by Protein G Affinity Chromatography.
ImmunogenFusion protein containing amino acid residues 326-616 of human RANK (cytoplasmic domain)
SpecificityRecognizes an epitope located in the cytoplasmic domain between amino acid residues 330-427 of human RANK.
Species crossreactivityMouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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