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You are here:Home » Growth Factors, Cytokines » Growth Factors-RANK » RANKL, Recombinant, Human (Receptor Activator of Nuclear Factor kappa B Ligand, Receptor Activator of NFkB Ligand, CD254, hRANKL2, hRANKL-2, Osteoclast Differentiation Factor, ODF, Osteoprotegerin Ligand, OPGL, sOdf, SOFA, TNF-related Activation-induced C

RANKL, Recombinant, Human (Receptor Activator of Nuclear Factor kappa B Ligand, Receptor
Activator of NFkB Ligand, CD254, hRANKL2, hRANKL-2, Osteoclast Differentiation Factor,
ODF, Osteoprotegerin Ligand, OPGL, sOdf, SOFA, TNF-related Activation-induced C

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Specifications

The soluble form of human CD254, otherwise known as sRANKL, is a 20kD glycoprotein and member of the Tumor Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein.
CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors. Binding of CD254 to receptor activator of NF-KBeta (RANK) is essential for the activation of osteoclastogenesis, whereas binding to the antagonistic decoy receptor osteoprotegerin (OPG) neutralizes CD254, thereby inhibiting osteoclastogenesis.
Studies have shown that CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation in the mixed lymphocyte reaction.
Catalog #R1075-10H
SourceRecombinant human RANKL expressed in E. coli
ActivityDose dependent stimulation of IL-8 production by human PBMC produced an ED50 of 10.0ng/ml.
ApplicationsSuitable for use in Functional Assays. Other applications not tested.
Recommended DilutionFunctional Assays: 0.2-20ng/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight20kD
SourceRecombinant, human, expressed in E. coli
Purity~98% by SDS PAGE and HPLC analysis. Endotoxin: 1EU/ug.
FormSupplied as a lyophilized powder from TRIS pH7.6. Reconstitute with 10ul sterile dH2O
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCD254 Antigen


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