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You are here:Home » Growth Factors, Cytokines » Growth Factors-RANK » RANKL, aa85-245, Recombinant, Human (Receptor Activator of Nuclear Factor kappa B Ligand, Receptor Activator of NFkB Ligand, CD254, hRANKL2, hRANKL-2, Osteoclast Differentiation Factor, ODF, Osteoprotegerin Ligand, OPGL, sOdf, SOFA, TNF-related Activation

RANKL, aa85-245, Recombinant, Human (Receptor Activator of Nuclear Factor kappa B Ligand,
Receptor Activator of NFkB Ligand, CD254, hRANKL2, hRANKL-2, Osteoclast Differentiation
Factor, ODF, Osteoprotegerin Ligand, OPGL, sOdf, SOFA, TNF-related Activation

Pricing

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Specifications

RANK L, is a 39-45kD type II transmembrane (TM) protein in the tumor necrosis factor family, designated TNFSF11 (1-5). RANK L, produced by osteoblasts and bone marrow stromal cells, is required for differentiation of osteoclasts and stimulates bone resorption (4, 6). It is also produced by activated T cells and augments dendritic cell stimulation; RANK L-/- mice lack lymph nodes and have impaired thymocyte development (1-3, 6). The human RANK L cDNA encodes 317aa, including a 47aa cytoplasmic domain, a 21aa TM region, and a 249aa extracellular domain (ECD) with two potential N-linked glycosylation sites (note: aa85-245 of Accession # AAC51762 is identical to aa157-317 of SwissProt # O14788. This aa range contains the ECD trimerization and receptor-binding motifs, but not ECD proteolytic cleavage sites). Within the ECD, human RANK L shares 89%, 89%, 93% and 95% aa identity with mouse, rat, bovine and porcine RANK L, respectively. Mouse RANK L can stimulate human osteoclast differentiation (4). Like most TNF family members, RANK L can form trimers (1). Soluble 31, 25 and 24kD forms of RANK L can be created by usage of alternate start sites at aa74 or 146, or proteolytic cleavage by osteoblast- or stromal cell-derived ADAM10 (after aa139) or MMP14 (146aa), or bone metastatic prostate tumor-derived MT1-MMP (aa146) (5, 7, 8). Both TM and soluble extracellular RANK L act by engaging RANK receptors and are antagonized by the decoy receptor, OPG (osteoprotegrin) (2, 5). In resting cells, the majority of RANK L is stored in secretory lysosomes (9). In mammary epithelia, RANK L is upregulated by pregnancy hormones and is essential for the formation of a lactating mammary gland (10). In the brain, astrocyte RANK L mediates body temperature regulation (11). Pathologically, RANK L is thought to mediate post-menopausal osteoporosis, vascular calcification, progestin-induced breast cancer, cancer-induced bone disease, and osteopetrosis (in RANK L deficiencies) (12-16).
Catalog #R1075-10T1
SourceRecombinant corresponding to aa85-245 from human RANK L, at N-terminal Met expressed in E. coli.
Molecular Weight~18.3kD
Endotoxin Level 1EU/1ug (LAL)
Biological ActivityMeasured by its ability to induce osteoclast differentiation on RAW 264.7 mouse macrophages.
The ED50 for this effect is typically 2-12ng/mL
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~18.3kD
SourceE. coli
Purity~90% (SDS-PAGE)
ConcentrationNot determined
FormSupplied as a lyophilized powder in sodium dihydrogen phosphate, sodium chloride, EDTA, BSA. Reconstitute with sterile PBS to 100ug/ml, 0.1% human or bovine serum albumin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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