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You are here:Home » Antibodies » Abs to RANK » Anti -RANKL (TNFSF11)

Anti -RANKL (TNFSF11)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IC
RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of RANKL and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated RANKL may have a role in the regulation of cell apoptosis. Targeted disruption of the related protein in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy (1).
Catalog #R1075-17
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000-1:10,000
Immunoprecipitation: 1:10-1:100
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
Clone NoEPR4999
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues at the C-terminus of human RANKL.
SpecificityRecognizes human RANKL. Species crossreactivity: Mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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