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You are here:Home » Molecular Biology » Growth Factors-Ligands » Rank Ligand, Recombinant Human

Rank Ligand, Recombinant Human

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Specifications

Recombinant Human soluble RANKL produced in E. coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton.
Catalog #R1075-77
Endotoxin <0.01ng/ug or <0.1EU/ug as per LAL
Biological ActivityRANK ligand is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml and by dose dependent stimulation of IL-8 production in human PBMC which was found to be <10ng/ml.
AA SequenceMEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight20kD
SourceRecombinant, human, E. coli
Purity98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
FormSupplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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