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You are here:Home » Growth Factors, Cytokines » Growth Factors-RANK » sRANKL, Recombinant, Human (soluble RANK Ligand, Receptor Activator of NFkB Ligand)

sRANKL, Recombinant, Human (soluble RANK Ligand, Receptor Activator of NFkB Ligand)

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Specifications

RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Catalog #R1076-03
Recombinant Human soluble RANKL is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Met.
Biological ActivityRANK ligand is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration of 5-10ng/ml and by dose dependent stimulation of IL-8 production in human PBMC.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight20kD
SourceE. coli
Purity~ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from 10mM Tris, pH 7.5. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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