RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Recombinant human RANTES is a 7.8kD protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC-chemokines.
Amino Acid Sequence
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
Endotoxin
≤ 0.1ng/ug (1EU/ug)
Biological Activity
Determined by its ability to chemoattract human blood monocytes using a concentration range of 1-10ng/ml.
Responding Cells (partial list)
Monocytes
Concentration Range for most in vitro applications
0.2-20ng/ml
Reconstitution
Lyophilized RANTES is soluble in water and most aqueous buffers. Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile dH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥ 98% by SDS-PAGE and HPLC analyses.
Form
Supplied as a lyophilized powder without additives.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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