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You are here:Home » Molecular Biology » Growth Factors-RANTES » RANTES, Recombinant, Human (Regulated on Activation, Normal T-cell Expressed and Secreted)

RANTES, Recombinant, Human (Regulated on Activation, Normal T-cell Expressed and Secreted)

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Specifications

Recombinant Human Rantes produced in E.Coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7809.2 Dalton. Recombinant Human Rantes is purified by proprietary chromatographic techniques.
Catalog #R1100-05A
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Tyr-Ser-Ser.
Biological ActivityED50 Range = 5.0-2.0ng/ml, determined by the dose dependent chemotaxis of THP-1 cells.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH2O, carrier protein. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight7.81kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O, carrier protein to 100ug/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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