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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Rap1 (Krev-1, smg p21)

Anti -Rap1 (Krev-1, smg p21)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Rap1, which is a member of the Ras family of GTP-binding proteins, cycles between an active GTP-bound and an unactive GDP-bound form that is mediated by GTPase activating protein (GAP). Rap1 is proposed to regulate Ras-mediated signaling and may also be involved in the regulation of Integrin-mediated cell adhesion although the mechanism of regulation is not known.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Catalog #R1120-10
Recommended DilutionsWestern Blot (ECL): 1ug/ml detects Rap1 in 20ug of rat brain tissue extract.
Optimal dilutions to be determined by the researcher.
Positive ControlsRat and Mouse Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to a portion of human Rap1a. Peptide differs from Rap1b by two residues.
SpecificityRecognizes human Rap1 at ~25kD. Species Crossreactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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