Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IH FC

RIAM (a Rap1-GTP-interacting adapter molecule) defines a family of adaptor molecules that contain a RA-like (Ras association) domain, a PH (pleckstrin homology) domain, and various proline-rich motifs (1). RIAM directly binds to Ena/VASP proteins, important modulators of cell migration that localize to focal adhesions and stress fibers, and co-localizes with them at focal adhesions in response to Ras activation. Moreover, RIAM directly binds to activated Ras in a phosphoinositide-dependent manner. RIAM is believed to be the first direct link between Ras signalling and cytoskeletal remodelling via Ena/VASP proteins during cell migration and spreading (2).
Catalog #	R1120-14
Applications	Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:50
	Western Blot: 1:1000-1:2000
	Immunohistochemistry: 1:100-1:250
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E519
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% Glycerol.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to residues near the N-terminus of human RIAM.
Specificity	Recognizes human RIAM at ~110kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.