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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -RIAM

Anti -RIAM

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH FC
RIAM (a Rap1-GTP-interacting adapter molecule) defines a family of adaptor molecules that contain a RA-like (Ras association) domain, a PH (pleckstrin homology) domain, and various proline-rich motifs (1). RIAM directly binds to Ena/VASP proteins, important modulators of cell migration that localize to focal adhesions and stress fibers, and co-localizes with them at focal adhesions in response to Ras activation. Moreover, RIAM directly binds to activated Ras in a phosphoinositide-dependent manner. RIAM is believed to be the first direct link between Ras signalling and cytoskeletal remodelling via Ena/VASP proteins during cell migration and spreading (2).
Catalog #R1120-14
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Western Blot: 1:1000-1:2000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E519
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% Glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human RIAM.
SpecificityRecognizes human RIAM at ~110kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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