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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -Raptor (Regulatory Associated Protein of mTOR, p150 Target of Rapamycin (TOR) Scaffold Protein containing WD-repeats, KIAA1303)

Anti -Raptor (Regulatory Associated Protein of mTOR, p150 Target of Rapamycin (TOR)
Scaffold Protein containing WD-repeats, KIAA1303)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Catalog #R1171-01C
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1,000-2,500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control Testing Immunoblot Analysis A 1:1,000-2,500 dilution of the lot detected endogenous Raptor in lysate from 293T cells. Immunoprecipitation Analysis: Reported by an independent laboratory to immunoprecipitate endogenous Raptor. Immunoblot Analysis: Lysates from 293T cells were resolved by electrophoresis, transferred to PVDF (Immobilon-P), and probed with anti-Raptor for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates Raptor.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in 0.02M PBS, 0.25M sodium chloride containing 0.1% sodium azide and 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenEptide encompassing the N-terminal region of human Raptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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