| Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates. |
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| Catalog # | R1171-01K |
| Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1ug/ml detected Raptor on 10ug of HEK293 cell lysate. HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with R1171-01K. |
| Proteins were visualized using a goat anti-mouse IgG (HRP) and chemiluminescence detection system. |
| Immunoprecipitation: 20ug |
| Immunocytochemisty: 1: 500 detected Raptor in NIH/3T3 cells. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| Positive Control | HEK 293 cell lysate. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1k |
| Clone No | 10F657 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% sodium azide. |
| Purity | Purified |
| Immunogen | Recombinant synthetic peptide corresponding to human Raptor. |
| Specificity | Recognizes human Raptor. Species Crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
