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You are here:Home » Antibodies » Abs to Protein Kinase Inhibitors » Anti -Raptor (Regulatory Associated Protein of mTOR, p150 Target of Rapamycin (TOR) Scaffold Protein containing WD-repeats, KIAA1303)

Anti -Raptor (Regulatory Associated Protein of mTOR, p150 Target of Rapamycin (TOR)
Scaffold Protein containing WD-repeats, KIAA1303)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.
Catalog #R1171-01K
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml detected Raptor on 10ug of HEK293 cell lysate. HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with R1171-01K.
Proteins were visualized using a goat anti-mouse IgG (HRP) and chemiluminescence detection system.
Immunoprecipitation: 20ug
Immunocytochemisty: 1: 500 detected Raptor in NIH/3T3 cells.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Positive ControlHEK 293 cell lysate.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1k
Clone No10F657
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% sodium azide.
PurityPurified
ImmunogenRecombinant synthetic peptide corresponding to human Raptor.
SpecificityRecognizes human Raptor. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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