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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -RasGAP (Ras GTPase Activating Protein, GAP)

Anti -RasGAP (Ras GTPase Activating Protein, GAP)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC IF
RasGAP, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGAP is a Caspase 3 substrate and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases. At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N. Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and Cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGAP promotes apoptosis.
Catalog #R1198-10E
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionsWestern Blot: 1:2000
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k345
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the SH3 domain of human RasGAP
SpecificityRecognizes human RasGAP. Species Crossreactivity: rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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