You are here:Home
»
Antibodies
»
Abs to GTPase, Rab, Ras, Rho Proteins
»
Anti -RASGRP3 (Ras Guanyl-releasing Protein 3, Calcium and DAG-regulated Guanine Nucleotide Exchange Factor III, Guanine Nucleotide Exchange Factor for Rap1, GRP3, KIAA0846)
Anti -RASGRP3 (Ras Guanyl-releasing Protein 3, Calcium and DAG-regulated Guanine
Nucleotide Exchange Factor III, Guanine Nucleotide Exchange Factor for Rap1, GRP3,
KIAA0846)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E |
|
| Lymphocyte activation occurs in part through activation of the Ras signaling pathway following lymphocyte receptor stimulation. The RasGRP family of guanine nucleotide exchange factors (GEFs) catalyzes the exchange of GDP for GTP on Ras family small GTPases, promoting their active GTP-bound form. Diacylglycerol (DAG) or phorbol ester binding to RasGRP family members causes their translocation to the cell membrane and stimulates their activity. While T-cells express RasGRP1, B-cells express both RasGRP1 and RasGRP3. RasGRP3 is important in linking B-cell receptor (BCR) activation to Ras signaling. In response to BCR stimulation, RasGRP3 is phosphorylated at Thr133 by PKC. This phosphorylation event further activates RasGRP3 in response to DAG, which stimulates PKC activity. | | | Catalog # | R1198-10G2 | | Applications | Suitable for use in ELISA. Other applications not tested. | | Recommended Dilution | ELISA: 1:64,000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to GSSGLGKAATLDE, from N-terminus of the protein sequence according to NP_733772. | | Specificity | Recognizes human RASGRP3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
