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You are here:Home » Antibodies » Abs to Ras-GTPase » Anti -RasGRP3, phosphorylated (pT133)(RASGRP3, KIAA0846)

Anti -RasGRP3, phosphorylated (pT133)(RASGRP3, KIAA0846)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IC
The RasGRPs are a family of Ras activators that possess diacylglycerol-binding C1 domains (1). RasGRP3 is an activator for H-Ras, R-Ras and Ras-associated protein-1/2, and has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells (2). It has been demonstrated that RasGRP3 undergoes phosphorylation at Thr-133 upon B cell receptor cross-linking, thereby resulting in its activation. Moreover, mutation of Thr-133 in RasGRP3 alone severely impaired its ability to activate Ras in B cell receptor signaling (3).
Catalog #R1198-10G3
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000-1:10,000
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
Clone NoEPNCIR148
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenA phospho-specific peptide corresponding to residues surrounding Threonine 133 of human RasGRP3.
SpecificityRecognizes human RasGRP3 phosphorylated at Threonine 133. Species crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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