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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -RasGAP (RAS p21 protein activator (GTPase activating protein) 1, CMAVM, CM-AVM, DKFZp434N071, GAP, GTPase-activating protein, p120GAP, p120RASGAP, PKWS, RASA1, RASGAP, Ras GTPase-activating protein 1, Ras p21 protein activator)

Anti -RasGAP (RAS p21 protein activator (GTPase activating protein) 1, CMAVM, CM-AVM,
DKFZp434N071, GAP, GTPase-activating protein, p120GAP, p120RASGAP, PKWS, RASA1, RASGAP,
Ras GTPase-activating protein 1, Ras p21 protein activator)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Ras-GTPase-activating protein SH3-domain-binding protein; GAP binding protein; RasGAP-associated endoribonuclease G3BP: This protein is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Catalog #R1198-11
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2.5ug. Immunoprecipates RasGAP from 500ug of A431 cell lysate.
Western Blot: 0.5-2ug/ml. Detects GAP in 10ug of A431 cell lysate.
Immunocytochemistry: 10ug/ml. Detects RasGAP on A431 cells fixed with ethanol acetic acid (50:50).
Optimal dilutions to be determined by the researcher.
Positive Antigen ControlA0001-50A: A431 Cells, Non-Stimulated in RIPA Buffer, w/o Sample Buffer
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Also Available Ras ProductsEnzymes:
R0495-15: Raf 1 RBD, Recombinant, Human (Agarose)
R1198-20: Ras, GST (Agarose)
AntibodiesR1198: Ras, p21, N,H,K Mab Mo xHu
KitsR1198-07: Ras Activation Assay Kit, BioAssay™
Ha-Ras ProductsAntibodies:
R1198-34: Ras, H (Harvey Murine Sarcoma Virus Oncogene, Ha-Ras) Mab Rb xHu
RasGAP ProductsAntibodies:
R1198-11: Ras, GAP (Ras GTPase Activating Protein, GAP) Mab Mo xHu
R1198-12: Ras, GAP (Ras GTPase Activating Protein, GAP) Pab Rb xHu
Clone TypeMonoclonal
IsotypeIgG
Clone No0.T.131
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in Tris-glycine, pH 7.2, containing sodium azide. Reconstitute with sterile 40-50% glycerol, ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenFull length recombinant human RasGAP expressed in sf9 insect cells.
SpecificitySpecific for human RasGAP (120kD). Species Crossreactivity: rat, mouse, hamster and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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