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Abs to GTPase, Rab, Ras, Rho Proteins
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Anti -Ras, p21 (neuroblastoma RAS viral (v-ras) oncogene homolog precursor )
Anti -Ras, p21 (neuroblastoma RAS viral (v-ras) oncogene homolog precursor )
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP FC |
|
| Ras, a proto-oncogene, is a small G-protein that has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. H-Ras was first discovered as a transforming product the retrovirus Harvey murine virus and K-Ras of Kirten sarcoma virus. Ras is a heavily studied target of both academic and pharmaceutical research because of its implications in various pathways and diseases as well as being mutated in a large number of human cancers. Ras is most notably the activator of the Erk/MAPK kinase pathway as activator of Raf, as well as an activator of PI3 Kinase (PI3K). In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways including the MAPK pathway through its activation of Raf, but also others as well that include PI3 Kinase and Ral GDS.C6 cell lysate | | | Catalog # | R1198-11B | | Applications | Suitable for use in Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:100 | | Western Blot: 1:500,000 | | Immunoprecipitation: 1:30 | | Optimal dilutions to be determined by the researcher. | | Positive Control | C6 cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9i196 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to residues near the N-terminal region of human Ras. Cellular Localization: Cytoplasm. | | Specificity | Recognizes H-Ras, N-Ras and K-Ras at and around the N-terminus. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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