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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Ras, p21 (neuroblastoma RAS viral (v-ras) oncogene homolog precursor )

Anti -Ras, p21 (neuroblastoma RAS viral (v-ras) oncogene homolog precursor )

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC
Ras, a proto-oncogene, is a small G-protein that has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. H-Ras was first discovered as a transforming product the retrovirus Harvey murine virus and K-Ras of Kirten sarcoma virus. Ras is a heavily studied target of both academic and pharmaceutical research because of its implications in various pathways and diseases as well as being mutated in a large number of human cancers. Ras is most notably the activator of the Erk/MAPK kinase pathway as activator of Raf, as well as an activator of PI3 Kinase (PI3K). In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways including the MAPK pathway through its activation of Raf, but also others as well that include PI3 Kinase and Ral GDS.C6 cell lysate
Catalog #R1198-11B
ApplicationsSuitable for use in Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100
Western Blot: 1:500,000
Immunoprecipitation: 1:30
Optimal dilutions to be determined by the researcher.
Positive ControlC6 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i196
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues near the N-terminal region of human Ras. Cellular Localization: Cytoplasm.
SpecificityRecognizes H-Ras, N-Ras and K-Ras at and around the N-terminus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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