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Abs to GTPase, Rab, Ras, Rho Proteins
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Anti -RasGAP (Ras GTPase Activating Protein, GAP)
Anti -RasGAP (Ras GTPase Activating Protein, GAP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP |
|
| Ras-GTPase-activating protein SH3-domain-binding protein; GAP binding protein; RasGAP-associated endoribonuclease G3BP: This protein is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. | | | Catalog # | R1198-12 | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested | | Recommended Dilutions | Western Blot: 0.5-2ug/m. Detects GAP protein in a RIPA lysate of human Raji lymphocyte cells, rat L6 myoblasts lysate and mouse Wehi leukemia cell lysate. | | Immunoprecipitation: 5ug. Immunoprecipitates GAP protein in a RIPA lysate of human Raji lymphocyte cells. | | Optimal dilutions to be determined by researcher. | | Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb | | I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb | | I1904-46Q: IgG, H&L (HRP) Pab Gt xRb | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from 0.1M Tris-HCl, 0.15M sodium chloride, 0.05% sodium azide. Reconstitute with 200ul sterile distilled water. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Trp E-fusion protein containing aa171-448 of human GAP. | | Specificity | Recognizes human GAP at 120kD. Species Crossreactivity: Chicken, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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