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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -RasGAP (Ras GTPase Activating Protein, GAP)

Anti -RasGAP (Ras GTPase Activating Protein, GAP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Ras-GTPase-activating protein SH3-domain-binding protein; GAP binding protein; RasGAP-associated endoribonuclease G3BP: This protein is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Catalog #R1198-12
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested
Recommended DilutionsWestern Blot: 0.5-2ug/m. Detects GAP protein in a RIPA lysate of human Raji lymphocyte cells, rat L6 myoblasts lysate and mouse Wehi leukemia cell lysate.
Immunoprecipitation: 5ug. Immunoprecipitates GAP protein in a RIPA lysate of human Raji lymphocyte cells.
Optimal dilutions to be determined by researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 0.1M Tris-HCl, 0.15M sodium chloride, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenTrp E-fusion protein containing aa171-448 of human GAP.
SpecificityRecognizes human GAP at 120kD. Species Crossreactivity: chicken, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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